Cell Based Assays
Promega NanoBiT PPI Control Pair (FKBP, FRB), 1 each
The NanoBiT PPI Starter Systems provide the vectors required to create the LgBiT and SmBiT protein fusions, a PRKACA:PRKAR2A positive control pair and a negative control vector. Starter systems also include the Nano-Glo Live Cell Assay System.
Promega MultiTox-Glo™ Multiplex Cytotoxicity Assay
The MultiTox-Glo™ Multiplex Cytotoxicity Assay is a fluorescent and luminescent assay that measures the relative number of live and dead cells in a population.
Promega ENLITEN™ Luciferase/Luciferin Reagent
The ENLITEN rLuciferase/Luciferin Reagent is intended for the rapid and quantitative detection of ATP in liquid samples.
Promega Caspase-Glo™ 8 and Caspase-Glo™ 9 Assay Systems
Caspase-Glo™ Assay is a homogeneous luminescent assay that measures caspase-8 or caspase-9 activity.
Promega Glutamate-Glo Assay, 50ml
The Glutamate-Glo Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamate in biological samples. The assay couples glutamate oxidation and NADH production with a bioluminescent NADH detection system.
Promega ADP-Glo™ Kinase Assay + CDK9/CyclinK Kinase Enzyme System
Recombinant full-length human CDK9 and CyclinK proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family.
Promega TGF beta R2 Kinase Enzyme System, 1mg
Recombinant human TGF beta R2 (190-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TGF beta R2 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase.
Promega ADP-Glo™ Kinase Assay + CLK3 Kinase Enzyme System
Recombinant full-length human CLK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CLK3, also known as CDC-like kinase 3, encodes a serine/threonine-type protein kinase with a non-conserved N-terminal domain.
Promega ADP-Glo™ Kinase Assay + HPK1 Kinase Enzyme System
Recombinant human HPK1 (1-346) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HPK1 or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family.
Promega MLK1 Kinase Enzyme System, 1mg
Recombinant human MLK1 (1-433) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH(2)-terminal kinase (JNK) pathway.
Promega ADP-Glo™ Kinase Assay + MELK Kinase Enzyme System
Recombinant human MELK (1-340) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MELK or maternal embryonic leucine zipper kinase is a member of the Snf1/AMPK kinase family.
Promega PYK2 Kinase Enzyme System, 1mg
Recombinant human PYK2 (360-690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family.
Promega PAK3 Kinase Enzyme System, 1mg
Recombinant full-length mouse PAK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PAK3 is a member of the family of ser/thr p21-activating kinases that serve as targets for the small GTP-binding proteins Cdc42 and RAC.
Promega AMPK (A1/B1/G2) Kinase Enzyme System, 10microg
Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMP-activated protein kinase (AMPK) exhibits a key role as master regulator of cellular energy homeostasis.
Promega PKA Kinase Enzyme System, 2,500 units
Recombinant bovine cAMP-Dependent Protein Kinase (PKA), Catalytic Subunit, is expressed and purified from E. coli. PKA does not require cAMP for activity, and the gene sequence can be found at accession number NM_174584.